Real-time PCR or quantitative PCR (qPCR), due to its ability to provide rapid, specific and quantitative results, is often considered to be a gold standard technology. It allows you to determine the initial amount of DNA or RNA present in a sample.
Benefits of qPCR
Highest precision and sensitivity.
Removal of PCR efficiency bias.
Blood, cells, fresh, frozen or FFPE tissue, bone marrow, DNA, RNA, cDNA, …
We can design, validate and implement a wide range of qPCR tests, for many applications including:
Genetic variation analysis: Genotyping allows the detection of genetic variations in the genome that can induce changes in phenotypes or pathological modifications underlying a disease.
Gene expression analysis: this is the most common qPCR application. It measures the level of expression of one or more genes.
Detection of pathogens (viruses, bacteria, etc.) or parasites
Detection of contaminating DNA in cell lines
We utilize QuantStudio 5 and LigtCycler 480 qPCR instruments.