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Fluorescence in situ hybridisation

FISH is a powerful and fast technique to detect focal DNA sequence alterations in cells and tissues. Labelled DNA probes linked to fluorescent molecule hybridise to complementary sequence of the DNA. Thereafter, fluorescent spots are visualised and counted by fluorescence microscopy. This tool has an intermediate resolution between the karyotype and techniques of molecular genetics.

Main applications and advantages

Various types of probes available:

  • Locus specific probes
  • Centromeric probes
  • Telomeric probes
  • Whole chromosome probes

Various types of matrix:

  • Fixed metaphase and interphase cell preparations
  • Smears
  • Formalin-fixed paraffin-embedded tissue

Equipment

  • Metafer Scanning and Imaging Platform (MetaSystems)
  • ThermoBrite Elite System for FISH Slide Preparation (Leica)

Detection

Detection of copy number alterations or rearrangements of a specific genomic region.

Doctors and scientists can help you.