Services > Cytogenetics


Karyotyping

Conventional karyotyping allows the study and detection of numerical and structural abnormalities of chromosomes. This technique is based on the staining of condensed chromosomes to provide a photographic representation of all chromosomes. The staining targets specific chromosomal regions called cytobands. The classification and the detection of abnormalities is based on the visual inspection of these. This is a genome-wide exploration tool with limited resolution (> 5Mb).

Main applications and advantages

  • Verification of cell cultures showing unusual growth
  • Identification of the origin of a cell line
  • Track chromosomal changes
  • Check chromosomal profile at the start and at the end of an experiment
  • QC for cell biobanking
  • QC for cells used in routine
  • QC before derivation of cell lines
  • Identification and characterisation of clonal and subclonal cell populations

Starting material

Peripheral blood, cell culture, fresh or frozen tissue, bone marrow…

Detection

  • Microscopic genomic aberrations/rearrangements > 5Mb
  • Chromosomes abnormalities: aneuploidy, inversions, duplications/deletions and translocations

Equipment

Slides of metaphase cells are scanned using the Metafer platform (MetaSystems). After acquisition, all metaphases are analysed on the IKAROS karyotyping station.

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