•  The following conditions can affect the test precision: multiple pregnancy, woman with a chromosomal abnormality or overweight or history of cancer.
  
  
• This test is not validated for detection of foetal mosaic chromosomal abnormality, foetal microdeletion/duplication or a monogenic disease (such as cystic fibrosis or X-fragile). However, if they are detected they will be reported.
  
  
• The following conditions for the pregnant woman can affect the results: blood transfusion, transplantation, stem cell therapy due to the presence of circulating DNA from an origin different from the mother or foetus.

Placental DNA from blood sample

• Modified commercial NextFlex™ kit (BIOO Scientific)
• NextSeq and NovaSeq sequencers